This randomized, double-blind, controlled clinical trial will evaluate the effects of daily cocoa flavanol supplementation on vascular and inflammatory biomarkers in healthy adult men aged 30 to 75 years. Participants will be randomized to receive either a cocoa flavanol supplement or a nutrient-matched low-flavanol control product for 4 to 8 weeks. The study will assess changes in inflammatory biomarkers, endothelial function, oxidative stress markers, vascular measurements, and circulating endothelial progenitor cell-related markers. Optional assessments include flow-mediated dilation and single-cell RNA sequencing of peripheral blood mononuclear cells to explore mechanistic biological responses to cocoa flavanol supplementation.
Age range
30 Years – 75 Years
Sex
MALE
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A starting point for the conversation — always confirm anything about your own eligibility, costs, and care with the study team and your doctor.
Change From Baseline in Circulating C-Reactive Protein Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating Interleukin-6 Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating Vascular Endothelial Growth Factor A Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating Endothelin-1 Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating Stromal Cell-Derived Factor 1 Alpha Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating E-Selectin Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating Intercellular Adhesion Molecule-1 Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating Vascular Cell Adhesion Molecule-1 Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating C-C Motif Chemokine Ligand 4 Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating C-C Motif Chemokine Ligand 2 Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating Interferon-Gamma Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating Tumor Necrosis Factor-Alpha Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating Interleukin-10 Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating Interleukin-1 Beta Concentration
Timeframe: Baseline and Week 4
Change From Baseline in Circulating Leptin Concentration
Timeframe: Baseline and Week 4