Active — Not RecruitingNot Applicable

Evaluation of the Effect of Smoking on Pyroptosis in Periodontal Pathogenesis

Turkey (Türkiye)113 participantsStarted 2024-10-15

Plain-language summary

This study investigates the impact of smoking on pyroptosis and inflammatory signaling pathways in systemically healthy individuals with various periodontal conditions. The research compares smokers and non-smokers across three clinical categories, resulting in six study groups: Healthy Control, Gingivitis, and Periodontitis. The study specifically evaluates the levels of IL-1beta, IL-18, and NLRP3 through ELISA, and the gene expression of TLR1, TLR4, and TLR6 through RT-PCR, to understand how cigarette smoke modulates innate immune responses in the absence of other systemic diseases.

Who can participate

Age range

18 Years – 65 Years

Sex

ALL

See this in plain English?

AI-rewrites the medical criteria so a patient or caregiver can understand them. Always confirm with the trial site.

Inclusion Criteria: * Individuals who voluntarily agreed to participate in the study, * Smokers (gingivitis and stage 3/4 generalized periodontitis) and non-smokers (gingivitis and stage 3/4 generalized periodontitis) who applied to the Periodontology Clinical of Suleyman Demirel University Faculty of Dentistry, * Individuals without any systemic diseases and without using any medications that affect periodontal health were included. Exclusion Criteria: * Individuals who refuse to participate in the study or who cannot comply with the study protocol, * Individuals participating in another clinical trial concurrently, Individuals who have received periodontal treatment within the last 6 months, * Individuals who have used medications (antibiotics, anti-inflammatory drugs, etc.) that may affect periodontal parameters within the last 3 months, * Individuals with systemic diseases that may affect the periodontium, * Individuals with systemic diseases presenting with oral symptoms, * Individuals with malignant disease, * Pregnant or lactating women, * Individuals with psychological or physical disabilities, * Individuals with substance abuse, * Individuals with severe malocclusion, undergoing orthodontic treatment, using appliances, or using removable dentures, * Individuals with acute dental infection or pain (cavities, abscesses, etc.).

Questions worth asking your doctor

Bring these to your next appointment. They're a starting point for a shared conversation — not a sign you qualify or a recommendation to enrol.

1Based on my diagnosis and history, is this trial worth exploring for me — or is there a standard treatment we should try first?
2What does this trial's phase tell us about how much is already known about its safety and benefit?
3What would taking part actually involve for me — visits, tests, time, and travel?
4What are the known and possible risks or side effects I should weigh, and how would they be monitored?
5If this trial isn't the right fit, what other options or trials would you suggest I look into?

Generated to help you prepare — always confirm anything about your own eligibility and care with the study team and your doctor.

Questions for the trial coordinator

The trial coordinator is the person who runs the study day to day. These cover the practical side — logistics, costs, and what taking part would actually mean for your life. The study team confirms whether you meet the criteria; these are questions to ask, not a sign you qualify.

1What does taking part actually involve week to week — how many visits, where, and how long does each one take?
2What costs are covered by the study, and what might I have to pay for myself, including travel, parking, or time off work?
3What happens during screening, and what happens if the study team confirms I don't meet the criteria after those tests?
4Who pays for the scans, blood work, and other tests the trial requires — the study, my insurance, or me?
5How will being in the trial affect my regular care, and will my own doctor stay informed and involved?
6Can I leave the trial at any point if I change my mind, and what would happen to my care if I do?

A starting point for the conversation — always confirm anything about your own eligibility, costs, and care with the study team and your doctor.

What they're measuring

Relative mRNA expression levels of TLR1,TLR4 and TLR6

Timeframe: Baseline (At the clinical examination)

Protein concerntrations of NLRP3, IL-18 and IL-1β

Timeframe: Baseline (At the time of clinical examinat

Trial details

NCT IDNCT07563192

SponsorSuleyman Demirel University

Sponsor typeOTHER

Study typeOBSERVATIONAL

Primary completion2025-11-18

View on ClinicalTrials.gov More Periodontitis trials