Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups. Bacterial identification by Gram stain, culture and biochemical rections * analysisThe resistance pattern of the isolates will be detected by disc diffusion method. * phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.
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1 ISOLATION OF KLEB. by culture on macConkey agar
Timeframe: 6 months from January to may
Antibiotic sensitivity testing of different antibiotics according to clsi 25
Timeframe: 6 months from January to may
Effect of Ceftazidime-Avibactam on Carbapenemase-Producing Klebsiella Pneumoniae
Timeframe: 6 months from January to may
Detection of resistance gene in isolates using conventional pcr
Timeframe: 6 months from June to December
Detection of some virulence genes using conventional pcr
Timeframe: 6 months from June to December