CompletedNot Applicable

Effect of I-PRF Application on ALP and MMP-9 Levels in Teeth With Apical Periodontitis

Turkey (Türkiye)60 participantsStarted 2025-01-01

Plain-language summary

The aim of this study was to evaluate the effect of injectable platelet rich fibrin (i-PRF) injection, in addition to routine root canal treatment, on alkaline phosphatase (ALP) and matrix metalloproteinase-9 (MMP-9) levels in teeth with chronic apical periodontitis. A total of 60 patients were included and randomly divided into two groups: the control group and the experimental group (i-PRF). Gingival crevicular fluid (GCF) samples and periapical exudate (PAE) samples were collected from the teeth at the first appointment, after completion of chemomechanical preparation. In both groups, calcium hydroxide was placed in the root canals, and the teeth were temporarily sealed with composite resin. While no additional procedure was performed in the control group, patients in the experimental group received submucosal i-PRF injection. The second samples were obtained after 4 weeks, and in the same session, the root canal treatment was completed, followed by permanent restoration. ALP and MMP-9 levels in the collected samples were analyzed using the ELISA test.

Who can participate

Age range18 Years – 64 Years

SexALL

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AI-rewrites the medical criteria so a patient or caregiver can understand them. Always confirm with the trial site.

Inclusion Criteria: * Sixty patients who applied to Kırıkkale University Faculty of Dentistry and were diagnosed with chronic apical periodontitis and were included in the PAI 3-4 groups according to the Periapical Index Scoring System (PAI) among the patients who were prescribed root canal treatment were included in the study. * Patients aged 18-64 * Upper or lower incisors and premolars with primary apical periodontitis * Patients with ASA I * Patients who have provided written informed consent to participate in the study. Exclusion Criteria: * Patients with ASA II or higher, * Patients who have used antibiotics and anti-inflammatory medications within the last month, * Patients with a periodontal pocket greater than 3 mm in the affected tooth, * Teeth with internal or external root resorption, or teeth with swelling and pain on palpation, * Pregnant women or those suspected of pregnancy, * Patients with generalized periodontitis, * Patients with immunosuppressive systemic diseases such as diabetes and hepatitis will not be included in the study.

What they're measuring

ALP and MMP-9 Levels in Periapical Exudate (PAE) and Gingival Crevicular Fluid (GCF)

Timeframe: Baseline and 28. Days

Trial details

NCT IDNCT07320898

SponsorKırıkkale University

Sponsor typeOTHER

Study typeOBSERVATIONAL

Primary completion2025-06-01