CGF-Enhanced Autologous Pulp Transplantation in Mature Necrotic Teeth

Inclusion Criteria: * Systemically healthy patients aged 15 to 40 years. * Mature permanent single-rooted teeth with clinical and radiographic diagnosis of pulp necrosis (or irreversible pulpitis with necrotic findings) associated with periapical radiolucency. * Probing depth \< 3 mm and no abnormal tooth mobility. * No previous endodontic treatment or apical surgery on the study tooth. * Availability of an autologous donor tooth (impacted or nonfunctional third molar indicated for extraction) to obtain pulp tissue. * Ability and willingness to attend follow-up visits and provide written informed consent. Exclusion Criteria: * Systemic conditions that may impair healing (e.g., uncontrolled diabetes mellitus, autoimmune disease) or use of immunosuppressive therapy. * History of head and neck radiotherapy. * Pregnancy or lactation. * Poor oral hygiene or periodontal disease with probing depth ≥ 4 mm. * Teeth with root fracture, internal/external root resorption, severe canal calcification, or non-restorable crown destruction. * Inability to comply with follow-up schedule.