The Effect of Hydration Status on Substrate Oxidation at Rest and During Light and Moderate Exercise

Inclusion Criteria: * Subject is male or female * If female, subject is not pregnant (based on self-report) * If female, subject has stopped taking birth control medication or removed long-acting reversible contraceptive \>6 months at start of the study * If female, subject has normal menstrual cycle (21-35 days) * If female, subject is willing to track their menstrual cycle * If female, subject has a smartphone compatible for application to track menstrual cycle (female only) and/or wearable data (females and males) * Subject is 18-35 years of age, inclusive * Subject body mass index (BMI) is between 18.5-25 kg/m2 * Subject is at least recreationally active (engaged in moderate-intensity, intermittent, or steady-state exercise at least 3 days per week for at least 60 min at a time) * Subject is accustomed to cycling for at least 60 min * Subject does not smoke (or has quit for at least 6 months) * Subject is not taking medication that may interfere with the study (e.g., diuretics, laxatives, or weight loss drugs) * Subject has no health conditions that would interfere with the study as indicated on the general health questionnaire (GHQ) e.g. cardiovascular, renal, or metabolic diseases * Subject is not actively involved in weight loss intervention (dieting and/or anti-obesity medications) * Subject is not allergic to adhesives (e.g., medical tape) * Subject is willing to avoid alcohol consumption 24 hours prior to visit(s) * Subject is willing to fast overnight (\~8-12 hour…