The aim of this study will be to evaluate the biological reaction of direct three dimensional printed retainers. Primary objective is to evaluate bacterial growth and color stability between different thicknesses of 3d printed material used as a retainer . Secondary objective is to determine the optimal thickness for use in clear aligner fabrication based on biological performance.
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Bacterial growth measured using colony-forming unit (CFU) count on agar plates.
Timeframe: from day 0 (Baseline time ) to 14 days after insertion
Color change of 3D printed retainers measured using spectrophotometer; ΔE calculated at day 0 and day 14.
Timeframe: from day 0 (baseline time ) to 14 days after insertion
Norhan Ibrahim Eldsokee Master's Candidate, B.D.S