Effects of Different Timing of Brisk Walking on Human Behaviours

Inclusion Criteria: * Aged between 20 and 50 years, with a BMI ranging from 19 to 27 kg/m². * Classified as having "low" physical activity levels (750 Metabolic Equivalents / week) according to the International Physical Activity Questionnaire (IPAQ). * Engaged in moderate-intensity exercise for no more than 3 days per week and less than 30 minutes per session in the 3 months preceding the study. * Maintained a stable body weight (± 3 kg) during the 3 months prior to the main experiment. * Not taking any medications, supplements, or vitamins that might affect food intake, appetite, physical activity, weight loss, or metabolism. * Free from cardiovascular and metabolic diseases. * Not pregnant or planning to become pregnant. * Not currently on a diet or undergoing any specific dietary interventions (e.g., intermittent fasting, ketogenic diet). * No injuries or surgical wounds preventing physical activity. * Regular sleep patterns, averaging 7-9 hours per night, with no shift work. * No habits of heavy alcohol consumption or smoking. Exclusion Criteria: * Personal history of/existing diabetes, cardiovascular disease, metabolic disease or dyslipidaemia. * Taking medications that may influence lipid or carbohydrate metabolism or immune system function. * Unable to take part in exercise for any reason (e.g., injury or disability) or a positive response to any questions on the Physical Activity Readiness questionnaire (PAR-Q).