The influence of a 6000m ergometer test on the markers of gut injury, endotoxemia, iron status, stress and inflammation in elite rowers.
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I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury
Timeframe: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LBP (lipopolysaccharide binding protein) to measure endotoxin
Timeframe: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Claudin 3 to measure tight junction leakage
Timeframe: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LPS (lipopolysaccharide) to measure endotoxin
Timeframe: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Lactic acid to measure fatigue after the race
Timeframe: Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.
Ferritin to measure iron status
Timeframe: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
Transferrin to measure iron status
Timeframe: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
Hepcidin to measure iron status
Timeframe: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
IL-6 (Interleukin-6) to measure inflammation
Timeframe: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
HO-1 (Heme Oxygenase 1) to measure oxidative stress
Timeframe: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) to measure oxidative stress
Timeframe: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.