A Clinical Study on Co-transplantation of Autologous Limbal Stem Cells and Corneal Stromal Stem Cells to Repair Corneal Injury

Inclusion Criteria: * Chemical injury from acid or alkali exposure * Extensive limbal stem cell deficiency (greater than 180 degrees), with corneal stromal opacity not exceeding a depth of 250 μm * Corneal epithelial conjunctivalization and fibrosis, with neovascularization extending into more than half of the quadrants * Unilateral ocular involvement, with normal limbal stem cell function in the contralateral healthy eye * No significant improvement in ocular surface signs and symptoms after pharmacological or surgical interventions, stable for over three months Exclusion Criteria: * Bilateral corneal stem cell deficiency, such as in Stevens-Johnson syndrome, ocular cicatricial pemphigoid, or congenital aniridia * Subclinical limbal stem cell deficiency in the contralateral eye * Signs of corneal endothelial decompensation, such as corneal bullae or corneal edema * Presence of infectious inflammation on the ocular surface * Severe dry eye * Eyelid abnormalities requiring corrective surgery to restore normal anatomical structure * Presence of chronic dacryocystitis, cataracts, uveitis, diabetic retinopathy, retinal detachment, or other ocular diseases * Patients who have undergone cataract surgery or other intraocular procedures * Glaucoma patients requiring long-term topical ocular medications * History of corneal perforation in the affected eye * Severe primary cardiovascular, hepatic, renal, endocrine, or hematologic disorders, diabetes, or immune deficiency in the medi…