Through training, the investigators can adjust the composition and functions of gut microbiota. Exercise also alters the body's energy metabolism pathways, which, in turn, impacts the gut microbiota and its metabolic products. Understanding how the body utilizes and regulates energy during exercise is essential for enhancing athletic performance. This study aims to explore the relationships among post-exercise energy metabolites, gut microbiota, and their metabolic products, applying omics technologies in the field of sports science. This interdisciplinary research will contribute to fields such as exercise physiology, sports nutrition, exercise biochemistry, and sports medicine, providing innovative and practical research insights for advancing sports science in our country.
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Concentration of lactate
Timeframe: The testing time points are set as follows: before exercise, at the 5-kilometer mark during exercise, immediately after exercise, and at 5, 15, 30, and 60 minutes post-exercise.
Concentration of creatine kinase
Timeframe: The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of glucose
Timeframe: The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of creatinine
Timeframe: The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of blood urea nitrogen
Timeframe: The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of lactate dehydrogenase
Timeframe: The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of free fatty acid
Timeframe: The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of cortisol
Timeframe: The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
ood frequency questionnaire(FFQ)
Timeframe: Before exercise
Gut microbiota analysis
Timeframe: The testing time points are set as follows: before exercise, immediately after exercise, and at 24 and 48 hours post-exercise.
Untargeted metabolome analysis
Timeframe: The testing time points are set as follows: before exercise, immediately after exercise, and at 24 and 48 hours post-exercise.