Prospective Validation Study of the CD8+TEMRA Cells As a Prognostic Biomarker of Healing Outcome After Fracture

Inclusion Criteria: * male or female subjects * subjects \> 18 to 80 years of age at the time of screening * closed fractures * fractures of the humerus-shaft, forearm-shaft, femur and tibia * Subjects suffering monotrauma or comparable with monotrauma, due to comparable post-surgery mobilisation * osteosynthesis * subject has signed an informed consent form * legal capacity Exclusion Criteria: * cancer related fractures * periprosthetic fractures * known active Hepatitis B virus or Hepatitis C virus infection at screening * known human immunodeficiency virus (HIV) infection, severe uncontrolled inflammatory disease or severe uncontrolled autoimmune disease (e.g., ulcerative colitis, Crohn's disease * active malignancy or history of malignancy within 5 years prior to screening * known diagnosis of moderate to severe dementia based on subject's medical history or severe psychiatric disorder * known history of drug or alcohol abuse in the past 12 months, based on self-report or medical record * history of autologous/allogeneic bone marrow (BM) or solid organ transplantation * exposure to allogeneic cell-based therapy in the past or exposure to autologous cell therapy in the last 12 months before screening * pregnancy * subject is currently enrolled in an investigational device or drug trial, or has not yet completed a period of at least 30 days since ending other investigational device or drug trial(s). * subject is detained or institutionalized under a court order or admini…