Optimal Pediatric Heart Transplant Immunosuppression With MicroRNAs

Inclusion Criteria: * Age ≤ 18 years at time of transplant listing * Subject is within 10-50 days post-orthotopic heart transplant at time of enrollment. * Planned follow-up at the transplant center for a minimum of one-year. * Caregiver able and willing to comply with the study visit schedule, study procedures, and study requirements. Exclusion Criteria: * Recipient of a multi-organ transplant * History of prior solid organ transplant before the index heart transplant * Ongoing mechanical circulatory support or hemodynamic instability after transplant * Active infection requiring either a) hospitalization or b) treatment with antimicrobial drugs (does not include prophylaxis for infection or suppressive antibiotics given after transplant) * History of treated rejection prior to study enrollment * Inability to collect specified blood volume after enrollment and prior to 50 days post-transplant