Metabolic Control of Aging and Disease - the MetAGE Deep Phenotyping Cohort

Inclusion Criteria: Group 1 - Lean, young adults (n = 150) * Age 18 - 35 years * Body mass index (BMI) ≥ 18.5 and ≤ 24.9 kg/m2 (≥ 12 months) Group 2 - Overweight, young adults (n = 150) * Age 18 - 35 years * BMI ≥ 28kg/m2 (≥ 12 months) Group 3 - Lean, older adults (n = 150) * Age ≥ 60 years * BMI ≥ 18.5 and ≤ 24.9 kg/m2 (≥ 3 years) Group 4 - Overweight, older adults (n = 150) * Age ≥ 60 years * BMI ≥ 28 kg/m2 (≥ 3 years) * Subgroup A (n=75): with pre-existent cardiovascular disease defined as: history of myocardial infarction or evidence of coronary artery disease irrespective of revascularization status or history of ischemic or hemorrhagic stroke or presence of peripheral artery disease or heart failure with preserved ejection fraction (NYHA Class I-II). * Subgroup B (n=75): without pre-existent cardiovascular disease Group 5 - Nonagenerians Age ≥ 90 (n = 50) * Age ≥ 90 years * BMI ≥ 18.5 kg/m2 (≥ 3 years) Exclusion Criteria: Group 1 - Lean, young adults (n = 150) * Highly physical active (i.e. \> 5 times sporting activity / week with moderate to high intensity \[heart rate 140 - 200bpm\]) * Special diets (i.e. ketogenic diet and time-restricted eating) * Clinically significant metabolic or endocrine disorders * Claustrophobia * drug abuse, alcohol \> 15 drinks/week * Heart failure, cardiovascular disease, immunosuppressive therapies, chronic inflammatory diseases and active oncologic conditions * Metal implants that prohibit 3T MRI * Pregnancy or breastfeeding…