CompletedNot Applicable

The Incorporation of Dietary Protein-Derived Amino Acids in Duodenal Epithelium

Netherlands10 participantsStarted 2023-07-24

Plain-language summary

Rationale: Aging is accompanied by a blunted muscle protein synthetic response to protein ingestion.This anabolic resistance may be related to decreased postprandial amino acid release in the circulation, due to greater amino acid extraction by splanchnic tissues in older individuals. It has been suggested that extracted amino acids are utilized by intestinal epithelial cells for cell proliferation, generating new cells to maintain healthy mucosa. So far, there is no evidence that dietary protein-derived amino acids are taken up and incorporated in intestinal mucosal protein in vivo in humans. Furthermore, there is no evidence that this process is impacted by age. Objective: To assess the postprandial incorporation of dietary protein-derived amino acids in intestinal mucosal protein in healthy young and older males. Study design: Cross-sectional, non-therapeutic intervention study design Study population: 5 healthy, non-obese (BMI 18.5-30kg/m2) young adult males (age: 18-35y inclusive) and 5 community dwelling older males (age: 67+y). Intervention: Continuous intravenous stable isotope amino acid tracer infusion will be applied, in combination with oral ingestion of 20g intrinsically labelled milk protein, with plasma, muscle and duodenal mucosa biopsy samples collected at different time points throughout the experimental test day. Main study parameters/endpoints: The primary study outcome is the postprandial (0-5h) incorporation of dietary protein-derived amino acids in duodenal mucosal protein following the ingestion of 20g intrinsically labelled milk protein. Secondary study parameters include postprandial plasma availability of dietary protein-derived amino acids and fractional duodenal mucosal protein synthetic rate.

Who can participate

Age range18 Years

SexMALE

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Inclusion Criteria: * Male sex * Aged 18-35 years or 67+ years * Body mass index (BMI) between 18.5 and 30 kg/m2 Exclusion Criteria: * History of cardiovascular, respiratory, gastrointestinal, urogenital, neurological, psychiatric, dermatologic, musculoskeletal, metabolic, endocrine, haematological, immunologic disorders, allergy, major surgery and/or laboratory assessments which might limit participation in or completion of the study protocol, interfere with the execution of the experiment, or potential influence the study outcomes (to be decided by the principal investigator and responsible physician) * Major abdominal surgery interfering with gastrointestinal function (upon judgement of the principal investigator and responsible physician) * Use of medication which limit participation in or completion of the study protocol, interferes with the execution of the experiment, or potential influences the study outcomes (to be decided by the principal investigator and responsible physician) * Use of supplementation (i.e. vitamin, pre- and probiotic supplementation) within 14 days prior to testing * Administration of investigational drugs or participation in any scientific intervention study in the 14 days prior to the study, which may interfere with this study (upon judgement of the principal investigator and responsible physician) * Specific diet (e.g. vegetarian, vegan, gluten free, no diary) within the study period * Planning to lose weight during the study period * Lactos…

What they're measuring

Postprandial incorporation of dietary protein-derived amino acids in duodenal mucosal protein

Timeframe: 0-5 hours

Impact of age on postprandial incorporation of dietary protein-derived amino acids in duodenal mucosal protein

Timeframe: 0-5 hours

Trial details

NCT IDNCT06091852

SponsorMaastricht University Medical Center

Sponsor typeOTHER

Study typeINTERVENTIONAL

Primary completion2024-04-05