Microbial Load After Apical Enlargement in Asymptomatic Teeth With Periapical Lesion

Inclusion Criteria: * Patients between the ages of 18-65 * Patients complying with the ASA1 and ASA2 classification (American Society of Anesthesiology) * Patients who has molar teeth with radiolucent lesions in the periapical region * The volunteer himself/herself or his/her legal representative wants to participate in the research by signing the 'Voluntary Consent Form'. * Being able to tolerate the treatment physically and mentally Exclusion Criteria: * Patients who have taken antibiotics/corticosteroids in the last 3 months * Patients with systemic disease * Teeth that cannot be properly isolated with a rubber dam * Teeth without coronal sealing, * Teeth with periodontal pocket depth\>4 mm and teeth with crown / root fracture. * Individuals who continue or are considering starting orthodontic treatment during the research * Pregnant and lactating individuals * The volunteer himself/herself or his/her legal representative does not want to participate in the research by signing the 'Voluntary Consent Form'. * Teeth that are damaged enough to require post-core treatment.