Buoy Electrolyte Study on Hydration Status of Active Men and Women

Inclusion Criteria: * Male or female, \>18 to 45 years of age * Freely given written consent * Non-tobacco users * Negative pregnancy test in women of childbearing potential * BMI \< 35 kg/m2 * GFR \> 60 ml/min * No known underlying medical condition * Willing to refrain from EtOH for 24h prior to test day * Willing to refrain from strenuous exercise for 24 h prior to each test day * Acceptable to have one 8oz cup of coffee/liquid on the morning of the test, but must be consistent each visit * Without active infection of any kind * Engaged in exercise three or more hours per week Exclusion Criteria: * Abnormal creatinine (Cr \> 1.2). * Proteinuria / hematuria / glucosuria based on urine dipstick. * Diagnosed medical condition that would impede results (CHF, HTN, CAD, CKD, history of electrolyte abnormality). * Pregnancy * Use of diuretics within past 2 weeks * Obesity (BMI \> 35) * Active infection based on symptoms (bacterial or viral) * Hemodynamic abnormality at screening visit: Blood pressure less than 100/60 or greater than 140/90.