Change in Skin Surface Temperature at the Knee Area on Both Sides When Using Mild Moxibustion Manipulation at Mingmen Acupoint With Moxa Stick

Inclusion Criteria: * Healthy males and females were individual age between enough 18 and 30 years old. * BMI: 18.5 - 23 kg/m2 * Mental alertness, good contact, cooperation with researchers. * Had no psychiatric stress problem during moxibustion day (confirmed by answering the DASS 21 questionaire with stress point less than 15 points). * Had no history of cardio-vascular disease, diabetes, hyperthyroidism, hypothyroidism. * Vital sign within normal limits: * Heart rates from from 60 to 99 beats/minute. * Systolic blood pressure between 90 and 139 mmHg. * Diastolic blood pressure between 60 and 89 mmHg. * Breathing rate: 16 ± 3 times/minute. * Body temperature: 36.59 ± 0.43 ℃. * SpO2 from 95 to 100%. * Volunteers who agree to participate and sign the Informed Consent Form, following a detailed explanation of clinical trials. * Not currently participating in other intervention studies. Exclusion Criteria: * Having an inflammatory injury in the site of the skin to be investigated. * Medical history of asthma or allergy with moxa smoke. * Using heat therapy, for example massage, cupping therapy, acupuncture in the site of the skin to be investigated within 24 hours. * Applying chemical or pharmaceutical products to the site of the skin to be survey before conducting the study. * Other diseases that could affect or interfere with outcomes, including common cold or flu. * Playing sport 2 hours before the study. * Taking stimulants such as beer, alcohol, coffee and …