Using a randomised crossover design, nine weight-stable men, aged 18 - 40 years old, will be recruited via convenience sampling from the staff and student body of LJMU and local area. Participants will be asked to follow two 4-day (\~96 hours) periods of tightly controlled exercise energy expenditure (15 kcal/kg FFM/day \[cycling\]) and dietary intake (60 kcal/kg FFM/day) to compare a state of 'normal' energy availability (or energy balance; equivalent to 45 kcal/kg FFM/day) with concomitant 1: normal carbohydrate availability ('Normal'; \~60% of dietary intake from carbohydrate) and 2: low carbohydrate availability ('LCHF', \~1.5 g/kg carbohydrate per day, \~70 - 80% dietary intake from fat). This approximates the amount of carbohydrate consumed by an individual in a state of LEA through consuming 10 kcal/kg FFM/day with 50% of intake from carbohydrate, or \~1.5 g/kg/day of carbohydrate. In both experimental phases we will measure endocrine, metabolic and physiological parameters.
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Changes in blood bone turnover markers: ß-CTX (Bone Resorption)
Timeframe: Days 1, 2, 3, 4 & 5 per intervention
Changes in blood bone turnover markers: P1NP (Bone Formation)
Timeframe: Days 1, 2, 3, 4 & 5 per intervention
Changes in blood metabolites/hormones: Testosterone
Timeframe: Days 1, 2, 3, 4 & 5 per intervention