Microbiota in Chronic Anal Fissure and Its Association With Prognosis

Inclusion criteria

• ✓. Consecutive patients referred to the outpatient clinic at the Digestive Disease Centre, Bispebjerg Hospital in Denmark for botox injection for chronic anal fissure. Thus, the included patients will likely all have tried conservative treatment before referral, and are expected to represent a more homogeneous subset with longer standing disease, less spontaneous improvement, and a higher likelihood of being compliant with suggested therapy
• ✓. Consecutive patients with an indication for revision of the fissure and anal injection of botulinum toxin in general anaesthesia
• ✓. Healthy volunteers. These will be recruited among staff at the Digestive Disease Centre, Bispebjerg Hospital, Denmark.