Investigating the Circulating Sphingolipidome Response to a Single High-intensity Interval Training Session

Inclusion Criteria: * Female or male sex, * Aged between 20 and 29 years old, * Body Mass Index (BMI) between 18.5 and 24.9 kg/m2, * Meeting the World Health Organization (WHO) guidelines on physical activity, i.e. at least 150-300 minutes of moderate-intensity aerobic physical activity per week as well as muscle-strengthening activities on 2 or more days per week, * Clearance for physical activity according to the 2022 Physical Activity Readiness Questionnaire (PAR-Q+), * Regular menstrual cycle, * Informed consent as documented by signature. Exclusion Criteria: * Females with known pregnancy or breastfeeding, * Females with known polycystic ovary syndrome (PCOS), * Current exercise-limiting conditions of the lower limbs (e.g. tendinopathy, fractures or other musculoskeletal pathologies), * Known acute or chronic diseases: e.g. any active infectious diseases, any past or current malignant tumours, any lung diseases (e.g. bronchial asthma), any cardiometabolic diseases (e.g. arterial hypertension, diabetes, dyslipidaemia), any gastrointestinal diseases (e.g. coeliac disease, Crohn's disease, ulcerative colitis), any psychological disorders (e.g. depression, if medically diagnosed, anorexia, bulimia), any endocrinological diseases (e.g. all types of diabetes mellitus, hyper- or hypothyroidism), any nephrological diseases, any neurological disorders, * Current or past smoking, current or past psychoactive drug use (alcohol excluded here, see below), * Excessive alcohol consu…