Microscopic Skin Tissue Column Grafting Technique Using the Autologous Regeneration of Tissue System

Inclusion Criteria: * Age greater than or equal to 18 and age less than or equal to 70. * Skin loss from surgery, trauma, and/or burns. * Presence of one uniform contiguous wound ≥150cm2 * Sufficient non-injured healthy skin from which conventional STSG can be harvested to cover study and non-study sites. * Sufficient non-injured healthy skin from which the study device can be used to harvest MSTC to cover the study sites. * Study donor site has not been previously harvested. * Able and likely to follow-up, to have three consecutive appointments and appear for 6 month follow up visit. * Subject agrees to abstain from enrollment in any other interventional clinical trial while enrolled in this study. Exclusion Criteria: * Exposure of tendon, bone devoid of peritenon or periosteum, or other structures deemed non-graftable by the treating physician or study investigator. * Concomitant skin disease/infection at the recipient or donor sites. * Use of topical steroid, anti-metabolite, or other pharmacologic therapy on study site. * Use of systemic steroids in the past month. * Concurrent conditions that in the opinion of the investigator may compromise subject safety, study objectives or wound healing. * Current recipient of immunosuppressive therapy. * Current treatment with medication that inhibit/compromise wound healing. * Known history of malignancy. * Pregnancy or lactation * Prisoner * History of noncompliance -\>40% total body surface area involvement.