Diabetic Foot Ulcer (DFU) Biofilm Infection and Recurrence

Inclusion Criteria: * Male or Female, Age ≥ 18 * Willing to comply with protocol instructions, including all study visits and study activities. * Patient with an open Diabetic Foot Ulcer * Adequate arterial blood flow as evidenced by at least one of the following (for wounds below the knee): * TcOM \>30 mmHg * Ankle-brachial index at least ≥0.7 * Toe pressure \> 30 mmHg * TBI \> 0.6 mmHg Exclusion Criteria: * Individuals who are deemed unable to understand the procedures, risks, and benefits of the study. * Wounds closed or to be surgically closed by flap or graft coverage * Subjects with marked immunodeficiency (HIV/AIDS, or on immunosuppressive medications. * TcOM \< 30mmHg * Diabetics with a hemoglobin A1c \> 15 within 3 months prior to enrollment * Subject with autoimmune connective tissue disease * Ulcer size and location that does not allow the TEWL measurement per SOP * Pregnant women * Prisoners * Unable to comply with study procedures and/or complete study visits