Maternal & Offspring Microbiome Study

Inclusion Criteria: * \> 18 years of age * Singleton pregnancies * Recruit during the first trimester and up to 22 weeks of pregnancy. * Healthy subjects willing and able to provide blood, stool, vaginal swab, and breast milk samples. * Must be able to provide signed and dated informed consent. Exclusion Criteria: * Pre-pregnancy BMI greater than 40 * Blood pressure SBP: \> 160 mmHg -OR- DBP: \> 90 mmHg * Kidney disease * Liver disease * Anemia * Symptomatic gallstones * History of bariatric surgery * Acute disease at time of enrollment (i.e. flu or gastroenteritis). May delay sampling until subject recovers. * Chronic, clinically significant, unstable (unresolved, requiring on-going changes to medical management or medication) pulmonary, cardiovascular, gastrointestinal, hepatic or renal functional abnormality, as determined by medical history * History of active uncontrolled gastrointestinal disorders or diseases including: * Inflammatory bowel disease (IBD) including ulcerative colitis (mild-moderate-severe), Crohn's disease (mild-moderate-severe), or indeterminate colitis; * Irritable bowel syndrome (IBS) (moderate-severe); * Persistent, infectious gastroenteritis, colitis or gastritis, persistent or chronic diarrhea of unknown etiology, Clostridium difficile infection (recurrent) or Helicobacter pylori infection (untreated) * Any confirmed or suspected condition/state of immunosuppression or immunodeficiency (primary or acquired) including HIV infection. * Surgery of …