Purpose: To compare the effects of the density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in a study population with astheno-teratozoospermia. Materials and Methods: In this prospective study, the semen samples of the patients with astheno-teratozoospermia were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were used to fertilize the mature sibling oocytes. Semen parameters and embryo development on days 3 and 5 were assessed.
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Semen parameters after sperm preparation with either method: semen volume
Timeframe: up to 7 months
Semen parameters after sperm preparation with either method: sperm concentration
Timeframe: up to 7 months
Semen parameters after sperm preparation with either method: total motility
Timeframe: up to 7 months
Semen parameters after sperm preparation with either method: progressive motility
Timeframe: up to 7 months
Embryo development after sperm preparation with either method
Timeframe: up to 7 months