Kinetics of Nasal Cytokine Responses to Mechanical Stimulation

Inclusion Criteria: All groups * Male or female * 18 to 50 years of age * Willingness to comply with the study protocol and written informed consent * Subjects must have a standard health care insurance * Subjects must be available during the study period to complete all treatments and assessments Allergic Rhinitis * Moderate to severe allergic rhinitis to grass pollen for at least two seasons according to medical history * Skin prick test positive to grass pollen extract * Blood ImmunoCAP sensitisation to grass pollen (\>0.35 kU/L grass pollen extract specific IgE or higher as determined by UniCAP-FEIA) * No history of Asthma Allergic Asthma * Clinical history of asthma * Spirometry reversibility or PC20 methacholine/histamine \<8mg * Skin prick test positive to grass extract * Blood ImmunoCAP sensitisation to grass pollen (\>0.35 kU/L grass pollen extract specific IgE or higher as determined by UniCAP-FEIA) Non-Allergics * No history of Asthma * No history of allergy * Negative skin prick test to a common skin prick test panel including grass pollen * No ImmunoCAP sensitisation to to grass pollen (\<0.35 kU/L grass pollen extract specific IgE or higher as determined by UniCAP-FEIA) Exclusion Criteria: * Older than 50 or younger than 18 years * Active smoker * History of anaphylaxis * Any severe chronic, malignant or general disease * Treatment with systemic or topical (intranasal, inhaled, external) corticosteroids within the previous 2 months before the start of …