Randomized parallel study investing the effect of intake of different protein sources (whey, insect and pea) on the muscle protein synthesis. Activation of the signaling pathway leading to muscle protein synthesis is investigated by western blotting and Real time quantitative polymerase chain reaction (qPCR or PCR). Urine, blood and muscle is moreover investigated by metabolomics analysis.
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Expression and phosphorylation of signaling proteins and their mRNA by Western Blotting and PCR
Timeframe: 4 hours
Blod and urine metabolism by NMR
Timeframe: 4 hours
Muscle metabolism by NMR
Timeframe: 3 hours