Immune Response followingTdap Vaccine in Pregnancy

Inclusion Criteria for pregnant women (cohort 1): * age between 18-45 years * pregnant women who expected to have Tdap vaccine * Informed Consent Form signature Inclusion Criteria for non pregnant women (cohort 2): * age between 18-45 years * non pregnant women who expected to have Tdap vaccine * Informed Consent Form signature Exclusion Criteria (cohort 1 and 2): * any background immune diseases- autoimmune conditions or cancer. * women who take immunosuppressive/ immunomodulatory medications * a patient has received Tdap vaccine in 6 months prior to study entry. * no will to signed the Informed Consent Form.