The specific aims of the project are performing a pre-clinical study on a human model and evaluating the oxygenation and other status of human erythrocytes by spectrally-resolved third harmonic generation (srTHG) microscopy . By analyzing the partitioned third harmonic generation (THG) spectra of oxygenated and deoxygenated erythrocytes ex vivo, the investigators aim to provide a reference standard for quantifying oxygenation and molecular status in this project.
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Observe the extent of tumor growth via morphological information
Timeframe: 2 years
Observe the extent of tumor growth via spectral THG intensity distribution
Timeframe: 2 years
Change in spectral THG strength distribution
Timeframe: 2 years