Vitamin C affect the melanocytes function not the number while diode laser cause melanocytes destruction. Although diode laser and vitamin C have proved their effectiveness in depigmentation in previous studies, there are no published studies compared the effect of diode laser and vitamin C on melanocytes and melanosomes clinically and histologically.
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Dummett oral pigmentaion index (DOPI)
Timeframe: change from baseline to 1, 3 and 6 months
gingival brightness (ΔL)
Timeframe: change from baseline to 1, 3 and 6 months
histological mean area fraction of melanosomes
Timeframe: change from baseline to 6 months