Relationship Between Sperm Head Vacuoles and Sperm DNA Alterations in Infertile Men

Inclusion Criteria: * men * 18 years old or more * affiliated to social security * with isolated teratozoospermia or oligo-astheno-teratozoospermia according to the WHO guidelines (2010): * sperm concentration \< 15 million per ml or sperm number \< 39 million per ejaculate * progressive motility \< 35% * morphologically normal forms \< 50% according to the modified David's classification (Auger et al., 2001 * mobile spermatozoa selected by density gradient centrifugation \> 1 million, in previous spermograms * with normal constitutional karyotypes (46, XY) * signed an informed consent Exclusion Criteria: * with abnormal constitutional karyotypes * with a major alteration of sperm parameters, with mobile spermatozoa selected by density gradient centrifugation \< 1 million * under tutorship or guardianship by court order