Cardiovascular Assessment of the Effects of Tobacco and Nicotine Delivery Products

Inclusion Criteria: * All Groups: Age 18-50 * Can tolerate withholding their medications for two weeks at a time * Group 1: Active smokers * Group 2: Nonsmokers * Group 3: Active users of smokeless tobacco * Group 4: Active users of electronic cigarettes * Additional Inclusion Criteria for E-Cigarette Users: * Currently use ofe-cigarettes \> 5 times a day * Has used e-cigarettes for \>3 months * Additional Inclusion Criteria for Active Smokers: Currently smoke \>5 cigarettes per day ≥ 1 pack year Exclusion Criteria: * Exclusion Criteria for all subjects * Physician diagnosis of: * asthma * heart disease * hypertension * dyslipidemia * thyroid disease * diabetes * renal or liver impairment * glaucoma. * Unstable psychiatric condition (such as current major depression, history of schizophrenia or bipolar disorder) * current use of more than two psychiatric medications * Pregnancy or breastfeeding (by history) * Alcohol, opiate, cocaine, amphetamine or methamphetamine dependence within the past 5 years * BMI \> 35 and \< 18 * Current opiate, cocaine, amphetamine or methamphetamine use (by history or urine test) * Occupational exposure to smoke, dusts and fumes * Concurrent participation in another clinical trial * Unable to communicate in English * Additional Exclusion Criteria for Active Smokers: * Unable to hold marijuana for 1 week prior to each study visit. * Exhaled carbon monoxide (CO) \<10 ppm at each visit * Negative salivary cotinine test using a rapid-read, over the…