Cold Plasma for Dental Restoration and Caries Prevention

Inclusion Criteria: * subject must be Caucasian or Africa American, 18-35 years old, have written, completed, informed consent forms; * be generally healthy; * be able to participate in the study; * have no diagnosed periodontitis and pericoronitis; * have no active mucosal diseases such as aphthous ulcers and herpetic stomatitis. They must also agree to follow study instructions. The patients must meet the following specific entry criteria: * 1-5 untreated caries and at least one class II caries. Exclusion Criteria: * each subject must have no diagnosed diabetes mellitus or any other endocrine diseases; * no diagnosed cardiovascular diseases; * no diagnosed immune-compromised diseases, such as HIV and AIDS; * no other serious systemic diseases, such as cancer; * no antibiotic therapy in the past 6 months; * no use of non-steroidal anti-inflammatory drugs (NSAIDS) in the past 3 months; and * no reported use of illicit drugs.