Evaluation in the Treatment of Nosocomial Sepsis Comparing Polymerase Chain Reaction With Conventional Blood Culture.

Inclusion Criteria: * Patient 18 or more years old * Patient staying more than 48 hours in hospital * Patient with clinical suspicion of infection and presenting at leat two of the following criteria:temperature \> 38oC or \< 36oC, heart rate \> 90 beats/minute, respiratory rate \> 20 breaths/minute or PaCO2 \< 32 mmHg or leukocytosis \>12 000/μl or leukopenia \<4000/μl or normal white blood cell count with \>10% immature forms * Patient or responsible able to provide informed consent Exclusion Criteria: * Patient submitted to cardiovascular surgery in the last fifteen days * Patient or responsible are not able to provide informed consent