This laboratory study is collecting and storing tissue, blood, and bone marrow samples from young patients with cancer. Collecting and storing samples of tissue, blood, and bone marrow from patients with cancer to study in the laboratory may help doctors learn more about changes that may occur in DNA and identify biomarkers related to cancer.
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Establishment and banking of cell lines and/or xenografts from pediatric patients with cancer
Timeframe: Up to 14 years
Establishment of continuous cell lines, under carefully controlled conditions, from pediatric patients with cancer
Timeframe: Up to 14 years
Establishment of transplantable xenografts in immunocompromised mice from tumor cells that are difficult to establish as continuous cell lines in vitro
Timeframe: Up to 14 years
Creation of a bank of cell lines and generation of sufficient vials of cryopreserved cells for distribution to investigators with approved COG biology protocols
Timeframe: Up to 14 years
Characterization of cell lines from childhood cancers with respect to DNA PCR molecular HLA profile as a "fingerprint" of original cell line identity
Timeframe: Up to 14 years
Characterization of cell lines for the ability for sustained growth in tissue culture and/or as mouse xenografts
Timeframe: Up to 14 years
Characterization of cell lines for mycoplasma contamination
Timeframe: Up to 14 years
Characterization of cell lines for expression of molecular makers that confirm the tumor-type of the cell line and the immortal nature of the cells (telomerase) and the expression of molecular markers that may correlate with drug resistance
Timeframe: Up to 14 years