Part A = Purpose of study Part A of the BOLT study will focus on characterising the total and essential amino acid concentrations for 4 hours following the consumption of a single serving of: 1) commercially available high-protein yoghurt drink, 2) a micellar casein drink, and 3) a flavoured water-based placebo drink. This is called a bioavailability study. Part B = Purpose of study Part B of the BOLT study will examine the effect of a twice-daily ingestion of 1) high protein yoghurt drink (test product), or 2) flavoured water based drink (control drink) on biomarkers of 1) muscle damage/stress, 2) physical function, 3) self reported pain and muscle soreness, and 4) range of motion, sleep, and recovery assessments, at 24, 48, and 72 hours following acute muscle fatiguing exercise in 18-45-year-old male and female participants. Participants will be randomly allocated to the test product or control drink groups prior to beginning the first visit.
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Essential Amino Acids
Timeframe: Baseline (0 min), 15, 30, 45, 60, 90, 120, 150, 180, 210, and 240 min
Total Amino Acids
Timeframe: Baseline (0 min), 15, 30, 45, 60, 90, 120, 150, 180, 210, and 240 min
Muscle damage biomarker 1
Timeframe: Baseline (0 hr), post-fatigue exercise (1 hr), 24 hr, 48 hr, 72 hour
Muscle damage biomarker 2
Timeframe: Baseline (0 hr), post-fatigue exercise (1 hr), 24 hr, 48 hr, 72 hour
Inflammatory biomarker 1
Timeframe: Baseline (0 hr), post-fatigue exercise (1 hr), 24 hr, 48 hr, 72 hour
Inflammatory biomarker 2
Timeframe: Baseline (0 hr), post-fatigue exercise (1 hr), 24 hr, 48 hr, 72 hour
Stress biomarker 1
Timeframe: Baseline (0 hr), post-fatigue exercise (1 hr), 24 hr, 48 hr, 72 hour