First To Know Pregnancy Test - Clinical Evaluation Protocol

Inclusion Criteria: * Females whose pregnancy status is unknown (e.g., have not used urine test at home) * Age Selection - must meet one of the following age groups: * Cohort A1 - Pre-menopausal: ≥14 years of age and ≤ 40 years of age with menstrual cycles (cycle lengths of 25-35 days) * Cohort A2 - Peri-menopausal: ≥41 years of age and ≤ 54 years of age * Cohort A3 - Post-menopausal: ≥55 years of age * Not menstruating at the time of the initial study visit * Must be willing to complete all study activities, provide all specimens/samples, and conduct all required testing * Willing to provide information regarding the diagnosis of pregnancy or details from their standard of care follow-up visit after initial visit * Sexually active * Not using contraceptives * English/Spanish speaking Exclusion Criteria: * Males * Females \<14 years of age * Recent pregnancy within the past 6 months * Trophoblastic disease or non-trophoblastic neoplasms * Downs syndrome (trisomy 21) * People with limited or no reading skills * Amenorrhea not related to peri or post menopause * Use of medications or hormonal contraceptives that may interfere with hCG levels at the time of testing (e.g., Gonadotropins: Luteinizing hormone)