Microbiological Description of Sperm and Follicular Fluid Samples. Impact on IVF/ICSI Success Rates (NCT07187414) | Clinical Trial Compass
CompletedNot Applicable
Microbiological Description of Sperm and Follicular Fluid Samples. Impact on IVF/ICSI Success Rates
France50 participantsStarted 2024-01-09
Plain-language summary
The goal of this observational study is to quantify and identify bacteria that may be present in semen and follicular fluid samples on the day of the IVF-ICSI attempt.
The secondary objective is to :
* Quantify and identify bacteria that may be present in embryo culture media on day 6.
* Correlate microbiological analyses of sperm and follicular fluids with those of embryo culture media on day 6.
* Evaluate their impact on fertilization and blastulation rates
Who can participate
Age range18 Years – 59 Years
SexALL
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Inclusion Criteria:
* Man aged 18 to 59 who have provided a sperm sample after an abstinence period of between 2 and 7 days. Sperm prepared by density gradient on day 0. Negative sperm culture (\<6 months). No specific urogenital history (varicocele, cryptorchidism, testicular ectopia, etc.).
* Women aged 18 to 43 undergoing oocyte puncture with collection of follicular fluid on day 0. No endometriosis or hydrosalpinx. Negative vaginal sample (\< 6 months).
* Negative HIV, HBV, and HCV serology tests within the last year.
* Absence of urinary tract infections in men and sexually transmitted infections (HPV, HSV, HIV-1/2, C. trachomatis, Gonorrhea, Syphilis, etc.) (\<6 months).
* Absence of recent antibiotic or antifungal treatment (end of treatment \< 3 months) Absence of probiotic intake (\< 3 months).
* No history of chemotherapy or radiation therapy.
* Embryonic culture performed up to day 6 for all zygotes.
Exclusion Criteria:
* Homosexual patient couples or transsexual couples
* Patients treated for medically assisted procreation with a third-party donor
* Frozen sperm pellets (ejaculate or surgical)
* Donor sperm
* Sperm prepared by washing
* Abstinence period \<2 or \>7 days
* Fever \<3 months
* History of chemotherapy or radiotherapy.
* History of urinary tract infections in man (\< 6 months)
* History of sexually transmitted infections (HPV, HSV, HIV-1/2, C. trachomatis, gonorrhea, syphilis, etc.) (\<6 months)
* Specific urogenital history (varicocele, cryptorchid…
What they're measuring
1
Quantification and identification of bacteria present in semen and follicular fluid samples on the day of the IVF-ICSI attempt.