In a crossover design, 8 participants will receive caloric drinks containing stable isotopes. In one arm of the study, participants will consume 6 hourly drinks containing two stable isotopes. Repeated blood, breath, urine, and muscle biopsies will be taken. In the second arm, participants will consume 1 drink containing 75g glucose, labeled with two stable isotopes of glucose. Periodic blood and breath will be taken over three hours.
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Define obesity-related whole body protein efficiency based of the IAAO method
Timeframe: 0 - 6 hours observational period ingesting six hourly meals, within 1 week of preliminary testing. Baseline measures taken at --30 minutes.
Assessment of oral glucose tolerance based on a dual tracer technique
Timeframe: 0- 3 hour observational period. Within 1 week of the prospective arm. Baseline taken at -30 minutes.