Oral, fecal and conjunctival samples will be collected from participants and will be stored (at - 20 and - 80 â—¦C) until further processing. On Day 1 and Day 2, all couples will participate in an oral, conjunctival and gut microbiota composition study. Optometry examinations will be completed for each participant Three and Six months later, oral, conjunctival and gut microbiota composition will be analyzed again with the same protocol. The participants will complete a validated Persian version of Pittsburgh Sleep Quality Inventory (PSQI), Epworth Sleepiness Scale (ESS), Insomnia Severity Index (ISI), global sleep assessment questionnaire (GSAQ) and Ocular Surface Disease Index. Consecutive couples will undergo a standard ophthalmology exam and specific assessments for dry eye, including non-anesthetic Schirmer's test and fluorescein tear break-up time on Day 1 and Day 90.
See this in plain English?
AI-rewrites the medical criteria so a patient or caregiver can understand them. Always confirm with the trial site.
Sleep Pattern
Timeframe: 3 months later, sleep quality and quantity will be measured with Pittsburgh Sleep Quality Inventory
Optometry examinations
Timeframe: 3 months
Depression
Timeframe: 6 months later, will be measured using Beck Anxiety Inventory-II.
Anxiety
Timeframe: 6 months later, will be measured using Beck Anxiety Inventory