The goal of this randomized placebo controlled crossover trial is to determine effects of oral nicotine and caffeine pouches on anaerobic performance, autonomic function, cognition, and behavior in adult non-obese adult male volunteers between ages 21 and 40. Accordingly, the primary aims of the study are to determine the effects of acute oral nicotine pouches, oral caffeine pouches, or both, on measures of * anaerobic performance * cognition * cardiovascular and autonomic function * appetite and food intake. Researchers will compare oral nicotine pouches, oral caffeine pouches, both oral nicotine and caffeine pouches, and a flavor and color matched placebo to see if how they differentially affect measurements of * physical performance, * cognition, * cardiovascular and autonomic function * appetite and food intake. On four separate occasions, participants will be asked to place the oral pouches in their mouth and complete several anerobic cycling, cognitive, and balance tests while blood and salivary biomarkers, subjective appetite ratings, and cardiovascular and autonomic function measurements are collected. After each trial, participants will complete a 24-hr food record.
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Wingate Anaerobic Cycling Test
Timeframe: Baseline, 15, 16, 19, 20, 23, 24, 27, 28, 31, 32, and 60 minutes post-ingestion
Concentration of blood lactate
Timeframe: Baseline, 25, 32 minutes post-ingestion
Concentration of salivary cortisol
Timeframe: Baseline, 15, 25, 32 minutes post-ingestion
Concentration of salivary interleukin-6
Timeframe: Baseline, 15, 25, 32 minutes post-ingestion
Salivary catecholamine concentration
Timeframe: Baseline, 15, 27, 40 minutes post-ingestion
Profile of Mood States
Timeframe: Baseline, 15, 32 minutes post-ingestion
Balance
Timeframe: Baseline, 15, 32 minutes post-ingestion
Ratings of perceived exertion
Timeframe: Baseline, 15, 16, 19, 20, 23, 24, 27, 28, 31, 32, and 60 minutes post-ingestion
Hunger - Visual Digital Analog Scale (0-100)
Timeframe: Baseline, 15, 25, 32 minutes post-ingestion
Desire to eat - Visual Digital Analog Scale (0-100)
Timeframe: Baseline, 15, 25, 32, 60 minutes post-ingestion
Prospective Consumption of Food - Visual Digital Analog Scale (0-100)
Timeframe: Baseline, 15, 25, 32, 60 minutes post-ingestion
Fullness - Visual Digital Analog Scale (0-100)
Timeframe: Baseline, 15, 25, 32, 60 minutes post-ingestion
Food Intake - Digital 24-hour food record
Timeframe: 24 hours following the end of each visit
Blood Pressure
Timeframe: Baseline, 15, 19, 23, 27, 31, and 60 minutes post-ingestion
Heart Rate
Timeframe: Baseline, 15, 19, 23, 27, 31, and 60 minutes post-ingestion
Trail Making Test - Digital Cognitive Function Test
Timeframe: Baseline, 15, 32 minutes post-ingestion
Sustained Attention Test - Digital Cognitive Function Test
Timeframe: Baseline, 15, 32 minutes post-ingestion
Visual Working Memory Span Test - Digital Cognitive Function Test
Timeframe: Baseline, 15, 32 minutes post-ingestion
Stroop Test - Digital Cognitive Function Test
Timeframe: Baseline, 15, 32 minutes post-ingestion
Deary-Liewald Test - Digital Cognitive Function Test
Timeframe: Baseline, 15, 32 minutes post-ingestion
Cerebral Blood flow
Timeframe: Baseline, 15, 19, 23, 27, and 31 minutes post-ingestion