The goal of this clinical trial was to explore how adding Tulsi extract, a natural plant substance, to chewing gum affects the levels of Streptococcus mutans, type of bacteria that cause dental caries, in the saliva of children aged 4 to 11 years. Participants should not use removable prosthetics or orthodontic appliances and had had not used antibiotics in the 2 weeks preceding sample collection. We want to answer this main question: Can chewing gum with Tulsi extract significantly reduce the number of Streptococcus mutans bacteria in children's saliva? Participants tasks: * Children were asked to chew gum containing Tulsi extract for 5 minutes and then dispose of the gum. * Two salivary samples were collected from each child: one before gum chewing and another 30 minutes after disposing the gum. Researchers compared Streptococcus mutans counts in saliva before and after gum chewing to determine if there is a significant reduction in bacterial colonies.
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Salivary Streptococcus mutans Count
Timeframe: Assessment will be conducted at two specific time points for each participant: Before commencing gum chewing, and after 30 minutes following the conclusion of chewing Tulsi Gum for 5 minutes...