Stopped: Unavailability of Primary investigator
Analysis of embryonic cell-free DNA (cfDNA) present in the spent culture media (SCM) is a non-invasive alternative for preimplantation genetic testing for aneuploidies (PGT-A) that avoids the technical challenges and limitations of biopsy. Initial studies investigating this non-invasive PGT-A (niPGT-A) method reported variable concordance between cfDNA in SCM and the trophectoderm sample (\~ 30%-86%) and indicated a contribution from both the inner cell mass and trophectoderm to the cfDNA in SCM. This study aims to evaluate the use of the embryo culture medium as a source of genetic material for PGT-A and validate a niPGT-A protocol using cfDNA in SCM.
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General concordance between results for cfDNA in SCM and trophectoderm biopsies
Timeframe: 4 months
Discordance per chromosome between results for cfDNA in SCM and trophectoderm biopsies
Timeframe: 4 months
Concordance per chromosome between results for cfDNA in SCM and trophectoderm
Timeframe: 4 months
Sensitivity of niPGT-A using cfDNA in SCM
Timeframe: 4 months
Specificity of niPGT-A using cfDNA in SCM
Timeframe: 4 months