Transbronchial Mediastinal Cryobiopsy Combined With EBUS-TBNA in the Diagnosis of Mediastinal Lesions

Inclusion Criteria: * Age \>=15 years; * More than one mediastinal lesions with diameter \>= 1cm was detected by thoracic CT; * Indication of biopsy to identify the etiology, including recently discovered mediastinal lesions or combination of clinical respiratory symptoms and complicated lung lesions implicated by thoracic image ; * Completion of necessary preoperative laboratory examination and other examinations such as cardiac ultrasound or CTA when necessary, in order to exclude potential contradictions; * Informed consent form achievement. Exclusion Criteria: * Contradictions to bronchoscopy examination such as severe cardiopulmonary diseases, coagulation disorders, intolerance to anesthesia or endoscopic operation and so on; * Failure to detect the mediastinal lesions in ultrasonic landscape; * Cysts or abscess; * Requirements for additional procedures other than EBUS examination (such as endobronchial biopsy); * Psychiatric disorders or severe neurosis. * Participation in other clinical experiments in recent 3 months; * Any other conditions considered to be inappropriate to be involved in this study.