Shigella CVD 30000: Study of Responses to Vaccination With Shigella Vaccine

Inclusion Criteria: * Male or female of age 18-45 years * Provides written informed consent * Healthy, based on history, exam, and medications * Documented acceptable screening laboratory work, including: WBC, ANC, Hg, Platelets Creatinine, ALT, Bili Serum IgA HIV, HBsAg, HCV Negative for HLA-B27 (this criterion does not apply to cohort 4) Stool culture urinalysis * Passing score on Comprehension Assessment Tool (greater than or equal to 70 percent correct answers) * Agrees not to participate in another interventional clinical trial during the study period * For females of child-bearing potential, must agree to acceptable birth control, 4 weeks before enrollment and through 4 weeks after last vaccination or challenge * Available for a 12-day inpatient stay (this criterion does not apply to cohort 4) Exclusion Criteria: * Positive pregnancy test at screening or within 24 hours of study product dosing * Poor venous access, as defined by inability to obtain venous blood after 3 venipuncture attempts (this criterion does not apply to cohort 4) * Abnormal vital signs, defined as: Systolic BP greater than 150 mmHg or Diastolic BP greater than 90 mmHg Resting heart rate greater than 100 Oral temperature greater than or equal to 100.4 degrees F * Persons with IgA deficiency (serum IgA less than 70 mg per dL * Serum S. flexneri 2a LPS igG titer greater than or equal to 2500 * Received prior vaccines or had prior infection (natural or challenge) with ETEC or Shigella, within 5 y…