Pharmacokinetics of Neostigmine and Glycopyrrolate

Inclusion Criteria: * Male or Female * Age 18-70 years Exclusion Criteria: * Previous adverse reaction or hypersensitivity to electrical stimulation, * Known sensitivity to neostigmine or glycopyrrolate, * History of mechanical obstruction of the GI or urinary tract, * Myocardial infarction within 6 months of trial, * Malignant and/or Uncontrollable Hypertension Defined by a blood pressure reading of 160/100 mmHg or higher with or without taking 3 or more different classes of anti-hypertensive medications, * Organ damage (heart \& kidney) and/or TIA-CVA as a result of hypertension, * Known past history of coronary artery disease or bradyarrhythmia, * Symptomatic orthostatic hypotension * Deep brain stimulation * Pregnancy (women who are sexually active and of childbearing potential must utilize a method of contraception and agree to maintain a contraceptive method until completion of the study), * Lactating, nursing females * Inability to provide informed consent signaled by MoCA cognitive test score of 20 or less, * History of ingrown hair folliculitis, * Concurrent illness and fever, * Concurrent participation in a research study, * VA employee.