Briefly, participants will be assessed for their eligibility for the study using an oral glucose tolerance test, which involves drinking a sugary drink followed by a series of small blood samples. If eligible, participants will be asked to take part in a fitness test and then two trials which will involve walking on a treadmill at a steady pace. Before and after the exercise a small piece of muscle will be taken from their thigh under local anaesthesia, as well as several small blood samples. Muscle muscle samples will be assessed for lipid metabolites.
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Plasma fatty acids levels
Timeframe: A change in the amount of fatty acids in the blood from baseline, to every 15 minute interval from the ingestion of acipimox up until 2 hours 45 minutes. From this point, samples will be every 30 minutes until 3 hours 45 minutes terminates the trial.
Skeletal muscle Diacylglycerol (DAG) levels
Timeframe: A change from baseline DAGs to DAGS immediately post and 3 hours post exercise trial.
Plasma Glycerol levels
Timeframe: A change in the amount of fatty acids in the blood from baseline, to every 15 minute interval from the ingestion of acipimox up until 2 hours 45 minutes. From this point, samples will be every 30 minutes until 3 hours 45 minutes terminates the trial.
IMTG utilisation during exercise
Timeframe: A change in amount of IMTG within the muscle from baseline, to immediately post and 3 hours post the exercise intervention.