Epigenetic Regulation of Human Adipose Tissue Distribution

Inclusion Criteria: * Age 18-45 years inclusive; * BMI 23-35 kg/m2 inclusive; * HbA1C \<6.0%; * Weight stable (± 3 kg) during the 3 months prior to enrollment; * Women must be \> 9 months post-partum; * Able to provide written, informed consent. Exclusion Criteria: * Postmenopausal women * Women with an oophorectomy * Fasting plasma glucose \> 126 mg/dL, or diagnosis with Type 2 Diabetes (T2DM) * Untreated or symptomatic thyroid disease. * Aminotransferase or aspartate aminotransferase \> 3x upper limit of laboratory reference range, or known diagnosis of liver disease. * Creatinine \> 2x upper limit of laboratory reference range, or known diagnosis of kidney disease. * Uncontrolled hypertension (BP \> 140 systolic or \> 90 diastolic) * New onset (\<3 months on a stable regime) use of oral contraceptives or hormone replacement therapy. * History of drug or alcohol abuse (\> 3 drinks per day) within the last 5 years, or psychiatric disease prohibiting adherence to study protocol. Current drug use may be determine by plasma or urine drug screens. * History of cancer within the last 5 years (skin cancers, with the exception of melanoma, may be acceptable). * History of organ transplant. * Myocardial Infarction within the last 6 months. * Current treatment with blood thinners or antiplatelet medications that cannot be safely stopped for biopsy and testing procedures. * History of HIV, active Hepatitis B or C or tuberculosis * Presence of clinically significant abnormalities on…