This study will serve to determine how influenza infection alters the bacterial colonization patterns in the nasal and oropharyngeal compartments, and whether the immune response correlate with these alterations. The investigators will be determining bacterial composition and immune responses in the nose and oropharynx during influenza infection. Specific Aims Therefore, the overall aims of this study are as follows: 1. To identify baseline composition and kinetic changes in the nasal and oropharyngeal microflora and immune responses after administering intranasal live attenuated influenza virus (i.e., FluMist® vaccine) or saline mist to healthy subjects; 2. To identify nasal and oropharyngeal microbial composition and local immune responses during influenza infections and after resolution of infection, and correlate these changes with clinical outcomes.
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Changes in the bacterial composition (Bacterial "microbiome") over time
Timeframe: Changes from baseline at 1-2 weeks and 4-6 weeks after LAIV or saline spray
Changes in gene expression in the nasal lining of study participants
Timeframe: Changes from baseline at 1-2 weeks after LAIV or saline spray