Autologous Prefabrication of Body Surface Tissues/ Organs(e.g. Joint)

Inclusion Criteria: * With body surface tissue or organ defects (e.g., ear, nose, bone and joint) requiring reconstruction. Exclusion Criteria: * evidence of infection, ischemia, ulcer or other pathological changes within the prefabricated area which defined as not suitable for tissues regeneration * history of delayed healing, radiational therapy; * significant renal, cardiovascular, hepatic and psychiatric diseases; * significant medical diseases or infection (including but not limited to the carrier of hepatitis B virus or HIV); * BMI \>30; * history of any hematological disease, including leukopenia, thrombocytopenia, or thrombocytosis; * Evidence of malignant diseases * unwillingness to participate.